W 0016/03 (Sex dysfunctions/NICOX S.A.) 03-02-2004

Summary of Facts and Submissions

I. The applicant filed an international patent application PCT/EP 01/08733 comprising a set of 11 claims, the independent claim of which read as follows:

Claim 1:

"1. Use for the treatment of sex dysfunctions of one or more of the following classes of drugs:

A) salified and non salified nitric oxide donor drugs, of formula

A - X1 - N(O)z

wherein the meaning of the terms appearing in the formula is as defined hereunder;

C) nitrate salts of compounds inhibiting phosphodiesterases;

in the compounds of general formula:

A - X1 - N(O)z

z is an integer and is 1 or 2, preferably 2;

A = R(COXu)t and wherein t is an integer 0 or 1; u is 0

or 1;

X = 0, NH, NR1c wherein R1c is a linear or branched C1-C10 alkyl;

X1 is the following bivalent linking group:

FORMULA

wherein:

nIX is an integer in the range 0-3;

nIIX is an integer in the range 1-3;

RTIX, RTIX', RTIIX, RTIIX', equal to or different from each other are H or a linear or branched C1-C4 alkyl;

Y is an heterocyclic ring containing one or two nitrogen atoms, optionally one oxygen or sulphur atom, said saturated, unsaturated or aromatic ring having 5 or 6 atoms;

R of the radical A of formula A - X1 N(O)z is selected from the following groups:

Group I) wherein t = 1 and u = 1

Ia)

FORMULA

Ib)

FORMULA

wherein:

R1 is the OCOR3 group; wherein R3 is methyl, ethyl or a linear or branched C3-C5 alkyl, or the residue of an heterocycle having only one ring having 5 or 6. atoms which can be aromatic, partially or totally hydrogenated, containing one or more hetero-atoms independently selected from O, N and S;

R2 is hydrogen, hydroxy, halogen, linear or branched C1-C4 alkyl, linear or branched C1-C4 alkoxy; a linear or branched C1-C4 perfluoroalkyl, for example trifluoromethyl; nitro, amino, mono- or di-(C1-4) alkylamino;

nI is an integer 0 or 1;

group II) wherein t = 1, u = 1

IIa)

FORMULA

IIb)

FORMULA

wherein:

RII5 is H, linear or branched when possible C1-C3 alkyl; RII6 has the same meaning as RII5, or when RII5 is H it can be benzyl;

RII1, RII2 and RII3 can independently be hydrogen, linear or branched when possible C1-C6 alkyl, or linear or branched when possible C1-C6 alkoxy, or Cl, F, Br;

RII4 is RII1 or bromine;

IIb) is the residue of the 2-[(2-methyl-3- (trifluoromethyl)phenyl]amino]-3- pyridincarboxylic] acid and when the -COOH group is present it is known as flunixin; group III) wherein t = 1, u =1 and R is

FORMULA

wherein:

R2a and R3a are H, linear or branched when possible, substituted or not, C1-C12 alkyl or allyl, with the proviso that if one of the two is allyl the other is H; preferably R2a is H, C1-C4 alkyl, R3a is H; R1a is selected from

FORMULA (II)

FORMULA (XXI)

FORMULA (IV)

FORMULA (XXXV)

FORMULA (VI)

FORMULA (VII)

FORMULA (VIII)

FORMULA (IX)

FORMULA (X)

FORMULA (III)

IIID) R1a corresponds to the following formulas:

FORMULA (IIIa)

FORMULA (XXX)

FORMULA (XXXI)

FORMULA (XXXII)

FORMULA (XXXIII)

FORMULA (XXXVI)

FORMULA (XXXVII)

FORMULA (XII)

wherein the meanings are the following:

- when R1a is as defined in formula (IV), Ketoprofen residue: RIII1 is H, SRIII3 wherein RIII3 contains from 1 to 4 carbon atoms, linear or branched when possible; RIII2 is H, hydroxy;

- when R1a is as defined in formula (XXI), carprofen residue: Rxxio is H, linear or branched when possible alkyl from 1 to 6 carbon atoms, C1-C6 alkoxycarbonyl linked to a C1-C6 alkyl, C1-C6 carboxyalkyl, C1-C6 alkanoyl, optionally substituted with halogens, benzyl or halobenzyl, benzoyl or halobenzoyl;

Rxxi is H, halogen, hydroxy, CN, C1-C6 alkyl optionally containing OH groups, C1-C6 alkoxy, acetyl, benzyloxy, SRxxi2 wherein Rxxi2 is C1-C6 alkyl; C1-C3 perfluoroalkyl; C1-C6 carboxyalkyl optionally containing OH groups, NO2, amino; sulphamoyl, dialkyl sulphamoyl with C1-C6 alkyl or difluoroalkylsulphonyl with C1-C3 alkyl;

Rxxi1 is halogen, CN, C1-C6 alkyl containing one or more OH groups, C1-C6 alkoxy, acetyl, acetamido, benzyloxy, SRIII3 being RIII3 as above defined, C1-C6 perfluoroalkyl, hydroxy, C1-C6 carboxyalkyl, NO2, amino, mono- or di-alkyl-amino C1-C6; sulphamoyl, di-alkyl sulphamoyl C1-C6, or di- fluoroalkylsulphamoyl as above defined; or Rxxi together with Rxxi1 is an alkylen dioxy C1-C6;

- when R1a is as defined in formula (XXXV) tiaprofenic acid residue:

Ar is phenyl, hydroxyphenyl optionally mono or polysubstituted with halogen, alkanoyl and alkoxy C1-C6, trialkyl C1-C6, preferably C1-C3, cyclopentyl, cyclohexyl, cycloheptyl, heteroaryl, preferably tienyl, furyl optionally containing OH, pyridyl;

- when R1a is as defined in formula (II), suprofen residue, wherein R3a is H, R2a is methyl and X = O;

- when R1a is as defined in formula (VI), R is the residue of indoprofen when R2a = H and R3a = CH3; of indobufen when R2a is equal to H and R3a = C2H5; X = O;

- when R1a is as defined in formula (VIII), R is the etodolac residue when R2a = R3a = H and X = O;

- when R1a is as defined in formula (VII), R is the fenoprofen residue when R3a = H, R2a = CH3 and X = O;

- when R1a is as defined in formula (III), R is the fenbufen residue when R2a = R3a = H and X = O;

- when R1a is as defined in formula (IX), R is the flurbiprofen residue when R3a = H, R2a = CH3, X = O;

- when R1a is as defined in formula (X) R is the tolmetin residue when R2a = R3a = H, X = O;

in group IIID) R1a corresponds to the following formulas:

- IIIa), when R2a = H and R3a = CH3 the pranoprofen residue is obtained: Alpha-methyl-5H- [l]benzopyran-[2,3-b]pyridin-7-acetic acid; the preferred compound has R2a = H, R3a = CH3, u = 1 and X = O:

- (XXX), when R2a = H and R3a = CH3 the bermoprofen residue is obtained: dibenz[b,f]oxepin-2-acetic acid;

- (XXXI), when R2a = H and R3a = CH3, R is the radical of the CS-670 compound: 2-[4-(2-oxo-l-cyclo- hexyliden methyl) phenyl]propionic acid;

- (XXXII), when R2a = R3a H the Pemedolac residue is obtained;

- (XXXIII), when R2a = R3a = H the pirazolac residue is obtained: 4-(4-chlorophenyl)-1-(4-fluoro phenyl) -3-pyrazolic acid;

- (XXXVI), when R2a = H, R3a = CH3 the zaltoprofen residue is obtained; when the residue is saturated with an hydroxyl or amino group, or with the carboxylic function the compounds are known as dibenzothiepine derivatives;

- (XXXVII), when R2a = R3a = H the mofezolac residue is obtained: 3,4-di(p-methoxyphenyl)isoxazol-5- acetic acid;

- (XII), when R2a = R3a = H the bromfenac residue is obtained: 2-amino-3-(4-bromobenzoyl)benzeneacetic acid;

in group IV) wherein t = 1, u = 1, R is

FORMULA

wherein:

RIVd and RIVdl are at least one H and the other a linear or branched C1-C6, preferably C1 and C2 alkyl, or difluoroalkyl with the alkyl from 1 to 6 carbon atoms, C1 is preferred, or RIVd and RIVdl form together a methylene group;

RIV has the following meaning:

FORMULA (II)

FORMULA (X)

FORMULA (III)

wherein the compounds of group IV) have the following meanings:

- in formula (II):

RiV-ii is C1-C6 alkyl, C3-C7 cycloalkyl, C1-C7 alkoxymethyl, C1-C3 trifluoroalkyl, vinyl, ethynyl, halogen, C1-C6 alkoxy, difluoroalkoxy, with C1-C7 alkyl, C1-C7 alkoxymethyloxy, alkylthio methyloxy with C1-C7 alkyl, alkyl methylthio with C1-C7 alkyl, cyano, difluoromethylthio, phenyl- or phenylalkyl substituted with C1-C8 alkyl.

- formula (X) loxoprofen residue;

- in formula (III):

RiV-iii is a C2-C5 alkyl, optionally branched when possible, C2 and C3 alkyloxy, allyloxy, phenoxy, phenylthio, cycloalkyl from 5 to 7 carbon atoms, optionally substituted in position 1 by a C1-C2 alkyl;

Group V)

FORMULA (VII)

FORMULA (IX)

FORMULA (IV)

FORMULA (V)

FORMULA (III)

FORMULA (II)

FORMULA (LX)

Group VE)

FORMULA (X)

FORMULA (XI)

FORMULA (XIII)

FORMULA (XXXX)

FORMULA (XXXXI)

In group V):

- when R is formula (II), RVii is H or a linear or branched C1-C4 alkyl;

RVii-1 is RVii or a linear or branched C1-C4 alkoxy; Cl, F, Br; the position of RVii-1 being ortho, or metha, or para;

- when R is formula (V)

of which the residue of the known tenidap has been indicated;

- when R is formula (V) A = R and t = 0,

- when R is formula (VII), A is RCO, t = 1 u = 0 or A is R and t = 0;

- when R is formula (IX), A = R and t = 0, or A = RCO with t = 1 and u = 0;

- when R is formula (III) A = RCOO, t = 1 and u = 0 or 1; or t = 0 and A = R;

- when R is formula (IV) A = RCOO, t = 1 and u = 1;

- when R is formula (LX) and in (COXu)t u = t = 1 and X is oxygen, the precursor compound is sulindac;

- when R is formula (X) it is the meloxicam residue;

- when R is formula (XI) the residue is known as ampiroxicam when the termination is -CH(CH3)OCOC2H5;

- when R is formula (XIII) and the valence is saturated with H, the residue derives from lornoxicam;

- when R is formula (XXXX) and the valence is saturated with H the compound is known as paracetamol;

- when R is formula (XXXXI) and the valence is saturated with H the compound is known as tramadol."

Claims 2 to 11 relate to preferred embodiments of the claimed use.

II. By the communication dated 22 January 2003, the European Patent Office, acting as an International Searching Authority (ISA), invited the applicant pursuant to Article 17(3)(a) and Rule 40.1 PCT to pay 18. additional search fees.

Referring to documents (1) (WO 00/12076), (2) (Helund et al. The Journal of Urology, 1994, 151, 1107-1113) and (3) (US-A-6077841), the ISA found that the use in the treatment of sex dysfunctions of compounds comprising the structural element NO and also the use of nitrate salts of compounds inhibiting phosphodiesterase was known from the prior art documents (1) to (3), and inferred from this finding that there was lack of unity, the claims covering 19 different groups of inventions:

Group 1: claims 1, 7-11 (partially), 2-5 in as far as they relate to the use of compounds of formula A-X1- N(O)z in relation to the treatment of sex dysfunctions.

Group 2: claims 1, 6-11 (partially), 2-5 in as far as they relate to the use of nitrate salt of compound C1 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Group 3: claims 1, 6-11 (partially), in as far as they relate to the use of nitrate salt of compound C2 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Group 4: claims 1, 6-11 (partially), in as far as they relate to the use of nitrate salt of compound C3 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Group 5: claims 1, 6-11 (partially), in as far as they relate to the use of nitrate salt of compound C4 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Group 6: claims 1, 6-11 (partially), in as far as they relate to the use of nitrate salt of compound C5 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Group 7: claims 1, 6-11 (partially), in as far as they relate to the use of nitrate salt of compound C6 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Group 8: claims 1, 6-11 (partially), in as far as they relate to the use of nitrate salt of compound C7 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Group 9: claims 1, 6-11 (partially), in as far as they relate to the use of nitrate salt of compound C8 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Group 10: claims 1, 6-11 (partially), in as far as they relate to the use of nitrate salt of compound C9 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Group 11: claims 1, 6-11 (partially), in as far as they relate to the use of nitrate salt of compound C10 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Group 12: claims 1, 6-11 (partially), in as far as they relate to the use of nitrate salt of compound C11 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Group 13: claims 1, 6-11 (partially), in as far as they relate to the use of nitrate salt of compound C12 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Group 14: claims 1, 6-11 (partially), 2-5 in as far as they relate to the use of nitrate salt of compound C13 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Group 15: claims 1, 6-11 (partially), 2-5 in as far as they relate to the use of nitrate salt of compound C14 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Group 16: claims 1, 6-11 (partially), in as far as they relate to the use of nitrate salt of compound C15 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Group 17: claims 1, 6-11 (partially), in as far as they relate to the use of nitrate salt of compound C16 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Group 18: claims 1, 6-11 (partially), in as far as they relate to the use of nitrate salt of compound C17 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Group 19: claims 1, 6-11 (partially), in as far as they relate to the use of nitrate salt of compound C18 as defined in claim 6 in relation to the treatment of sex dysfunctions.

Claim 6 reads:

"6. Use according to claims 1-5, wherein the nitrate salts of the compounds inhibiting the phosphodiesterase are selected from the following: (C1) 1-[4-ethoxy-3-(6,7-dihydro-1-methyl-7-oxo-3- propyl-1H-pyra-zol[4,3-d]-pyrimidin-5-yl)- phenyl]sulphoyl]-4-methyl-piperazine (Sildena- fil), (C2) 2-(2-propyloxyphenyl)-8-azapurin-6-one (Zapri-nast), (C3) 2,6-bis-(diethanolamino)-4,8- dipiperidine py-rimido [5,4-d]-pyrimidine (dipyridamol), (C4) 6-chloro-4-(1,3-dioxaindan-5- yl)methylamino-2(4-carboxy-1-pyperidi-nyl)- quinazoline, (C5) N-(phenyl methyl)-1-ethyl-1H- pyra-zol-[3,4-b]-quinolin-4-amine, (C6) 1-(2- chlorobenzyl)-3-isobutyryl-2-propyl-6-amino carbonyl-indol, (C7) 1-benzyl-6-chloro-2-[1-[3- (imidazol-1-yl)propyl]indol-5-yl-amino carbonyl] benzimidazol, (C8) 2-(1-imidazolyl)-5-(phenyl)-4- (1,3-dioxaindan-5-yl) methyl aminopyrimidine, (C9) 6-ethynyl-4-(2-methoxyethyl)amino-2-(1- imidazolyl)quinazo-line, (C1O) 1-cyclopentyl-3- ethyl-6-(2-propoxyphenyl)py-razol [3,4- d]pyrimidin-4-one, (C11) 1-cyclopentyl-3-ethyl-6- (4-methoxybenzyl)-pyrazol-[3,4-d]-pyrimidin-4-one, (C12) 1,3-dimethyl-6-(2-propoxy-5- methansulphonamidophe-nyl)-1,5-dihydro pyrazol [3,4-d]-pyrimidin-4-one, (C13) (6R, 12aR)-2,3, 6,7,12, 12a-hexahydro-2-methyl-6-(1,3-dioxan-5- yl)pyrazine [2',1':6,1] pyrido [3,4-b]indol-1,4- dione, (C14) 1-propyl-3-methyl-6-[2-propoxy-5- [(4'-me-thyl-1-pyrazinyl)sulphonamido] phenyl]- 1,5-dihydropyra-zol[3, 4-d]pyrimidin-4-one, (C15) 3-(4-amino carbonyl-1-piperidinyl)-6-cyan-8-(3- chloro-4-methoxy-phthalazine, (C16) 2-(1- imidazolyl)-4-(1,3-dioxaindan-5-yl) methyla-mino- 7,8-dihydro-5H-thiopyran[3,2-d]pyrimidine, (C17) 1-Cyclopentyl-3-ethyl-6-(3-ethoxypyrid-4-yl)-1H- pyrazolo [3, 4-d]pyrimidin-4-one, (C18) 1-[3-[1- [(4-Fluorophenyl) methyl]-7,8-dihydro-8-oxo-1H- imidazo[4,5-g]quinazolin-6-yl]-4-propoxyphenyl] carboxamide."

III. By its reply faxed 19 February 2003, the applicant paid one additional search fee under protest pursuant to Rule 40.2(c) PCT.

In support of the protest, the applicant argued in its "Statement under Rule 40.2(c)" that documents (1) to (3) did not disclose whether the use of the claimed compounds has a more advantageous therapeutic profile than sildenafil citrate (ie reduced effect on systemic pressure) as required and shown by the claimed invention according to the description of the application (page 2, lines 19-21 and page 25, table 1), so that claims 1 to 11 complied with the requirement of unity of invention.

IV. In a prior review pursuant to Rule 40.2(e) PCT dated 23. June 2003, the ISA found the invitation to pay additional fees to be justified and invited the applicant to pay the protest fee. In substance the Review Panel also considered that, in the light of documents (1) to (3), the presence of the structural element NO in compounds to be used for sexual dysfunction was already known.

V. By a letter of 10 July 2003, the applicant paid the protest fee according to Rule 40.2(e) PCT.